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SignaGen flag-tagged rab8a constructs
Flag Tagged Rab8a Constructs, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag-tagged+rab8a+constructs/flag+tagged+rab8a+constructs/pm29357897-138-21-28
Average 90 stars, based on 1 article reviews
flag-tagged rab8a constructs - by Bioz Stars, 2026-10
90/100 stars

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Cell Culture:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Construct:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Generated:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Transfection:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Staining:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Incubation:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

De-Phosphorylation Assay:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Expressing:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Mutagenesis:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Immunocytochemistry:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Stable Transfection:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Fluorescence:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Western Blot:

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation
Article Snippet: HEK293T cells were cultured as described and co-transfected at 80% confluence with 600 ng of GFP-Rabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.

Article Title: Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Article Snippet: HEK293T cells were cultured as described and cotransfected at 80% confluence with 600 ng of GFPRabin8 and 200 ng of flag-tagged Rab8a constructs using 6 μl of LipoD293 (SignaGen Laboratories) per well of a 6 well plate overnight in full medium.



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SignaGen flag-tagged rab8a constructs
Flag Tagged Rab8a Constructs, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag-tagged+rab8a+constructs/flag+tagged+rab8a+constructs/pm29357897-138-21-28
Average 90 stars, based on 1 article reviews
flag-tagged rab8a constructs - by Bioz Stars, 2026-10
90/100 stars
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